LOCUS       CO897675                 419 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04h06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O02080 O02080 SIMILAR TO CCAAT/ENHANCER-BINDING
            PROTEIN. [1], mRNA sequence.
ACCESSION   CO897675
VERSION     CO897675.1  GI:51237465
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 591
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aacagtcaca acacgaaaac gccaaaatgt ccgcccaacg gagcggccga aagcggaacg
       61 cggcacagca atcggcggac gaggcaactg gacaaccgca acagcagcac aactcacccg
      121 gacagcaatg gagtccggcg agcagcaacc aggagcagct ggacgagtac gcgttgaaga
      181 ggttgcgcaa caacgaggcg gtgagcagaa cgcgccagaa aaagcgcctg gagcaacagc
      241 acacctcgca acgcgtgaag gagctgcgag aggagaatgc ccagctggag aggacactgg
      301 acacaatgcg acgcgagttg gccctgctga aggagatggt ggtggtgtgt gcggccaagg
      361 gcgacgaggg gagcagcaac aggtgatgga tggtggcggt ggacgaacga attctatta
//