LOCUS       CO897672                 540 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04h03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q93576 Q93576 F25H2.5 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897672
VERSION     CO897672.1  GI:51237462
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tctcactttt aaatagccat ttatcgacgc aaatacgcga tgtctactaa aaaccaagaa
       61 cgcacattca tcgcgatcaa gcctgatggt gtccaacgcg gtcttgtgag caagataatc
      121 aaacaatttg agcagcgtgg ttataaactg gtggctatca aaatggtcaa agcaacgccc
      181 caacacttgg aagtgcatta tgaggatctg aaggacaaac ctttcttcgc tggattagtg
      241 aagtacatgg cttcagggcc agttgtggca atggtttggg aaggactcga tgttgtgaaa
      301 caaggtcgag ctatgctcgg agccacaaat ccactggtga gtgcagcagg aacaattcgt
      361 ggaagctact gtatggacat gggacgaaac atctgccacg gatcaaacag tgttccatcg
      421 gcagagcgtg aaattgcaca ctggttcaag caggaagaat tggttgactg ggagccagca
      481 cagatcaact ggatctacga gtgaagaaca atctggaaga cgcccatctg cgataaggaa
//