LOCUS       CO897671                 464 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04h01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9XWV7 Q9XWV7 Y37D8A.15 PROTEIN. [1] ;contains
            element LTR3 repetitive element, mRNA sequence.
ACCESSION   CO897671
VERSION     CO897671.1  GI:51237461
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cagtccgttc cgctctcact cactcaacaa aatgaagtcg tccgtcctcg tttccgctct
       61 gctgtgcgcc atctccgcac ttctgctccg agtccaggcc aacgcggcgc ccgatgcggc
      121 cagcgacttt ctagtgccgg tgccgttagt gtgtgcacag ctggccggaa gtgacgaaga
      181 gcgaatgctg ttgtgccaac tgtacgagtc gtcagccctg ctggcgcaat taggtgctct
      241 tgtgaacgag ggaatcggcc gactggcgac cagccaagga ttggccagag ccccgtcagt
      301 tgcagcggcg gacgctggcc gagagaagcg aaagcacgag tacttgcgct tcggcaagcg
      361 aaagcacgag tatctgaggt tcggcagaaa gtgaggcaaa gggcacaaag aaggtggaag
      421 gaactggcaa agcgagaaaa catcacatcc cctccaaaac tcaa
//