LOCUS       CO897669                 582 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04g09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YQ5A_CAEEL Q09253 HYPOTHETICAL 32.0 KD PROTEIN
            C16C10.10 IN CHROMOSOME III. [1], mRNA sequence.
ACCESSION   CO897669
VERSION     CO897669.1  GI:51237459
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 503.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 atgccaatca atgcccgttc ccttcactat gttttccgaa ttgccaaccg agaggcgtcc
       61 tacaagttct acaccgatct gctcggaatg aagatccttc gtcatgagga gttccacgaa
      121 ccatgccagg cttcgtgcaa tggcccgttc gatgggatgt ggagcaagac gatggtgggc
      181 tatggcagtg aggaggacca cttcgtgctg gagctgacct acaactacga agtgcactcc
      241 tacgagttgg gaaacgattt cgggggaatc cacatcgagt cggaggacgt gttccagaga
      301 gtggcggcgg aggcgcagcg acagcccgac gggactctgg cgctgagaga ccccgacggc
      361 cacgcgttct tcatcgggag gggctccgcc aaacaccccg tcaccagggt gtccgtgaac
      421 gttgtcgacc tggccaaatc cagggatttt tggtcgtctt tgctagaaat gaaggttgaa
      481 agtgcccccg aaagtgcctg tcttctgtcc tatggcgaag gtccatgcca acttgaactg
      541 aacccccttt caacgagtgc ctcatgggaa cgcaaatcag gc
//