LOCUS       CO897652                 491 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04e12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O73666 O73666 40S RIBOSOMAL PROTEIN S11, mRNA
            sequence.
ACCESSION   CO897652
VERSION     CO897652.1  GI:51237442
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 489.
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tccatttgaa ggatggccga acagactgaa cgcgcttacc aaaagcagcc cacggtgttc
       61 ctgaatgaca aattccgcac tcagggcgtt ggcaagaagc cggccaacaa agaccgatac
      121 tggaagaacg tcggtctcgg cttcaagacg ccgcgcgagg ccatcgaggg cacttacatt
      181 gacaagaagt gcccgttcac tggcaatgtg tccattcgag gccgcattct caccggggtg
      241 gtggtcaaga acaaaatgca gcggaccatc gtgatccgcc gagactacct gcactttgtg
      301 cccaagtacc gccgttacga gaagcgtcac aagaacatgt ccgtccattg ttcgccttgc
      361 ttccgtgacg tctccgtcgg agacattgtc actgttggag agtgccgccc gctgagcaag
      421 actgtccgct tcaacgtgct caaagtgatc aaggcgagcg gatcaaaaaa ggcattcgac
      481 aaattctgaa a
//