LOCUS       CO897650                 404 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04e10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:DPOA_RAT O89042 DNA POLYMERASE ALPHA CATALYTIC
            SUBUNIT, mRNA sequence.
ACCESSION   CO897650
VERSION     CO897650.1  GI:51237440
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 413
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttttctgtga ttgatcctat ttcaagtttg agagccggca acaacgacta caaagcacat
       61 gaagcaagtt gtcaatcgga actactcctt gctcgagttg gacattgacg acctttacaa
      121 gcgtatgcta ttgcacaaga agaagaaata cgcaccattg gtcgttgatt tgaacgacga
      181 gaatgttctc tcggaggaga agaagggact ggacattgtg gatctatagc tcaatcgaag
      241 cgttttgaca agctcattcg catggtacca catttaggcg gatctgatca atataaagtt
      301 taaaccaatt tatgacatgt caatctttca gccaaaactg ttgtaagcta tcaatttgtg
      361 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//