LOCUS CO897649 448 bp mRNA linear EST 12-JAN-2011 DEFINITION pe04e08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA 5' similar to SW:VATF_CAEEL Q23680 PROBABLE VACUOLAR ATP SYNTHASE SUBUNIT F, mRNA sequence. ACCESSION CO897649 VERSION CO897649.1 GI:51237439 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 448) AUTHORS McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J., Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B., Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C., Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C., Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T., Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R., Waterston,R. and Wilson,R. TITLE The Washington Univ. Nematode EST Project, 1999 JOURNAL Unpublished COMMENT Contact: Mitreva M WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library constructed by: Irina Ronko and Dr. Makedonka Mitreva Seq primer: SL1 primer. FEATURES Location/Qualifiers source 1..448 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1" /dev_stage="Mixed" /lab_host="GC10" /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI; Site_2: 3': EcoRI; The library was constructed by Irina Ronko and Dr. Makedonka Mitreva (mmitreva@watson.wustl.edu) at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Radopholus similis total RNA was provided by Dr. Godelieve Gheysen of Ghent University (lighe@gengenp.rug.ac.be)." ORIGIN 1 gggcaaattg ctagccgtca tcggcgacga agatacagtt gtagggttct tgctaggagg 61 cattggacag ctgaacaaag cacgcaaacc aaatttcctg atcgttgaca agaataccag 121 tattgcggaa atcgaggaag cattccggac tttttgcacg cgggacgaca tcgccatcat 181 actgatcaac cagcacatcg cggaaatgat ccggtaccaa gtagaccaac acgtggccag 241 cactccggca gtgctggaaa ttccctcgaa agagatgccg tacgacgcgt cgaaggacag 301 tatattgaac agggctcgtg gcctcatcaa tcctgaagac ctcaaataga gagtttcaaa 361 acgaactgat gaaacccatt tcattccgga tttcgacgaa ctattttcac attcacaact 421 atagaacgca aaaaatcgat ttttgtcc //