LOCUS       CO897649                 448 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04e08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:VATF_CAEEL Q23680 PROBABLE VACUOLAR ATP SYNTHASE
            SUBUNIT F, mRNA sequence.
ACCESSION   CO897649
VERSION     CO897649.1  GI:51237439
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gggcaaattg ctagccgtca tcggcgacga agatacagtt gtagggttct tgctaggagg
       61 cattggacag ctgaacaaag cacgcaaacc aaatttcctg atcgttgaca agaataccag
      121 tattgcggaa atcgaggaag cattccggac tttttgcacg cgggacgaca tcgccatcat
      181 actgatcaac cagcacatcg cggaaatgat ccggtaccaa gtagaccaac acgtggccag
      241 cactccggca gtgctggaaa ttccctcgaa agagatgccg tacgacgcgt cgaaggacag
      301 tatattgaac agggctcgtg gcctcatcaa tcctgaagac ctcaaataga gagtttcaaa
      361 acgaactgat gaaacccatt tcattccgga tttcgacgaa ctattttcac attcacaact
      421 atagaacgca aaaaatcgat ttttgtcc
//