LOCUS       CO897639                 461 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04c11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL34_SCHPO O42846 60S RIBOSOMAL PROTEIN L34. [1],
            mRNA sequence.
ACCESSION   CO897639
VERSION     CO897639.1  GI:51237429
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 473
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcaagtgcgc ttagaaaatg ccgcagcgtt tgacctatcg caggcgactc tgctacaaca
       61 caaagtcgaa caagaccaag gtggtcaaga caccaggagg ccgcaatgtg ttacactatc
      121 gcaataagct cggctccgtg cccaagtgtg gcgacactgg cgtcccgctg aaaggaatcc
      181 aagctgcgcg acctcgccaa ttggccaagc tgactaagcg aaagaagacc gtcacgcgtg
      241 catacggagg atgcctgagc gctgcagcag tgcgccagcg aataatccgc gcattcttgg
      301 tcgaggaaca gcagattgcg aacaagatcc tgaaagccaa gagtgctgga aggaagtaaa
      361 ccgatggaag gttttggttc caccatgttc tatttgttgt tgcattaaag tttgttctgt
      421 caaaaaaaaa aaaaaaaaaa taaaaaaaaa aaaaaaccaa a
//