LOCUS       CO897630                 424 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04b12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to WP:CE00739 F37C12.4 Ribosomal protein YL39, mRNA
            sequence.
ACCESSION   CO897630
VERSION     CO897630.1  GI:51237420
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 425
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 caaaaaagag aagggagcag acatgacgaa gcaccccgct gtcgaggccc ttgctgtggg
       61 actggagaaa ggctatacgg tgacaagaaa tgagcgcaag aagcgtcagt cgcggcacaa
      121 gggccgcctg tcgaagaaga cccgcattgt gcgggaactg gtgcgcgaag tggtcggctt
      181 tacgccgtat gagcgtcgca ccatggagct gttgcgcatc agcaaggaca agaaggctct
      241 caagttcctg aagaagcgca ttgggcaaca cgggcgcgca aagagaaagc gtgacgagat
      301 ccaacaagtg ctggtggcgc aacgcaaggc acacaaataa gaagacggac attgaaagcc
      361 ttgacgacca attgttggct attaccgaat cttgttaaac ataagtatat gaaaaaaaaa
      421 caaa
//