LOCUS CO897629 338 bp mRNA linear EST 12-JAN-2011 DEFINITION pe04b11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA 5' similar to SW:RL23_CAEEL P48158 PROBABLE 60S RIBOSOMAL PROTEIN L23. [1], mRNA sequence. ACCESSION CO897629 VERSION CO897629.1 GI:51237419 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 338) AUTHORS McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J., Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B., Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C., Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C., Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T., Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R., Waterston,R. and Wilson,R. TITLE The Washington Univ. Nematode EST Project, 1999 JOURNAL Unpublished COMMENT Contact: Mitreva M WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library constructed by: Irina Ronko and Dr. Makedonka Mitreva Seq primer: SL1 primer High quality sequence stop: 293. FEATURES Location/Qualifiers source 1..338 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1" /dev_stage="Mixed" /lab_host="GC10" /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI; Site_2: 3': EcoRI; The library was constructed by Irina Ronko and Dr. Makedonka Mitreva (mmitreva@watson.wustl.edu) at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Radopholus similis total RNA was provided by Dr. Godelieve Gheysen of Ghent University (lighe@gengenp.rug.ac.be)." ORIGIN 1 caacgtactt tccattcaaa atgtcgaagc gaggtcgtgg aggagcttcg ggagcgaaat 61 tccgtgtcac tttgggtctc ccagtcgggg ctgtaatgaa ttgcgccgat aataccggag 121 caaagaatct gtttgtcatt gccgtgtacg ggattcgcgg tcggcttaac cgacttccaa 181 gtgccgccgt cggagacatg tttgtgtgtt ccgtcaaaaa aggcaaacca cagctgcgca 241 aaaaagttct ccaagccgta gttgtccgcc agcgaaaaca atatcgtcgg aaggatggca 301 ctttccttta cttccaggac aacgccggaa ttatcatc //