LOCUS       CO897628                 511 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04b10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9Y5V0 Q9Y5V0 HSPC038 PROTEIN, mRNA sequence.
ACCESSION   CO897628
VERSION     CO897628.1  GI:51237418
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 560
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgtagagcca taaaagagcg aaatggcgcg gggccatcag aaagcacttt cgcagcagaa
       61 gaatcagcaa aagcaacagc agatgaagaa acagcagggc agtgaccaga aggcggctgc
      121 cgcaaaagca ctcaatttca aatgcgccgt atgcatggcg ttgatgcccg atccgaagac
      181 ctacaaacag cacttcgagt caaaacatcc aaagaacccg cttccgccag aattggccga
      241 tgtgcaggct taaaagcata atggatggat atgaagagtc gttcgtgaat gctggcggct
      301 gtcttcaatc ttgcgaaaaa atgaagtgtg ataaaaataa tttatgtaaa gataaacatt
      361 ttgttgggcc tcgtactgaa agaagaaact tacctatttg ccttctaaaa acgatcgctc
      421 gctttcactt tgtatcagtt tcgactttca aatgcccctc cctctggtcg ggagacattt
      481 atgcaattca ataaataata attatcgcaa a
//