LOCUS       CO897627                 389 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04b08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q17754 Q17754 C06G3.11 PROTEIN. [1], mRNA
            sequence.
ACCESSION   CO897627
VERSION     CO897627.1  GI:51237417
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 390
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 atgggtaatc cgacacagaa actgatctcc ggactttccg cgattttctg cttcatacaa
       61 cgcagttacg gagcaatgct tcaacgcgtg tgtagtggac tttacaggca ggaatatcaa
      121 cgagaaggag gagcggtgtt ccaagaattg tatggacaaa ttccttaagg ccggacaggt
      181 tgtcgatgcg tttccaggag tatcagttgc tcaacatgga ggcccaaggg gcgcctgttg
      241 cggccaccaa gaacgcgcaa ccgggaacgt gatactaggc atatgctagc gcccacacta
      301 cttgttaact tttcttttgc tcaatttttg aatagtccga acagcacaag taaagttgtt
      361 aaatagaaaa aaaaaaaaaa aaaaacaaa
//