LOCUS       CO897623                 476 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04b01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL10_MAIZE P45633 60S RIBOSOMAL PROTEIN L10, mRNA
            sequence.
ACCESSION   CO897623
VERSION     CO897623.1  GI:51237413
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttgattatgg gaaggcgtcc ggcacgctgt taccgtatac atcaagaaca agccgtaccc
       61 gtaagtcacg gttttgtcgt ggtgtgcctg acccagagat ccgaatcttc catctgggcc
      121 gaaaacgcgc caccgctcga ccaatttcca tgctgcgtgc atatgatttc caacgagcgt
      181 gaacatctat cgtctgaagc gcttgaagcc gcacgtattt gtgcaaacaa atacatggtc
      241 aagaattgcg gaaaagatgg cttccacatg cgtgtccgaa ttcatccgtt ccacgtgctt
      301 cgcatcaaca aaatgttatc atgcgccggc gctgaccgcc ttcagacggg gatgcgaggg
      361 gcctttggaa aaccgcaagg gcttgtggcc cgtgtcggaa tcggcgatgt gctgatgtca
      421 gtgcgtgtcc gtgaccaaca cgaggctcat gctctggaag ctttccgacg tgcaaa
//