LOCUS       CO897618                 491 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe04a04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q20634 Q20634 F52A8.5 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897618
VERSION     CO897618.1  GI:51237408
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 389.
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gtcgtctgct gctgttgaac tgttgctccg gcgcactgcg ctcggcagcc gccacgcgcc
       61 agcatagatc agcagcactt gtcacggctc gccaattctc cggctcacag tcacttctga
      121 acaaagtgca gcgcttttat acgcccaagc acgaatggat tgcactggag gagtccggga
      181 aggtcggcac tgtcgggatc accgactttg cgcaggacca actgggcgat gtggtgtacg
      241 tggagctgcc cgaggtgggc aagcagctaa gcgccggcga gacggcgggt gccatcgaaa
      301 gcgtcaaggc ggcctctgat ctgtacgcgc cagtcagcgg ccaagtggtg gagtgcaatg
      361 ccgaagtgga agaaaaaccc catctggtga aaaagcactg ctacaacaaa ggctggctgt
      421 acaaactgaa agtggcggac gcggcggaat tgaaagccct tgatggaaaa ggcgcttacg
      481 cggaagttaa g
//