LOCUS       CO897615                 488 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03h11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to contains element MER22 repetitive element, mRNA
            sequence.
ACCESSION   CO897615
VERSION     CO897615.1  GI:51237405
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 545
            Seq primer: SL1 primer
            High quality sequence stop: 471.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttaatccaaa aatccattca cgctgtcctt tccgcgacaa tcgaccacta aaatggccca
       61 gttcctgtac gcatttctcg cctccctact cctaggtgtg gtgctcttcg accgaaatgc
      121 gatgggacaa cagttcgcag agttcggggt tgccggccct gaaatgatgg acctggacta
      181 cggtagcatc aatgacattg agagccttgt gaagagagcc tcgttggacc gatcggcgat
      241 ggccagattc ggcaagcgcg caccgttgga ccggtcggcc atggccagat tcggcaaacg
      301 cgcgccactg gaccgatccg cgatggtcag attcggaaag cgagcccctc tcgaccgatc
      361 ggcaatggcc agattcggaa agtgaagtga cagagcgagc ggtgtggcgc attttttata
      421 aataagtgtg aaggaagcaa gcacaatcta acatgttcac acaaacacac tctttggatc
      481 aacagtta
//