LOCUS CO897614 453 bp mRNA linear EST 12-JAN-2011 DEFINITION pe03h10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA 5' similar to WP:CE25354 Y50D7A.I, mRNA sequence. ACCESSION CO897614 VERSION CO897614.1 GI:51237404 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 453) AUTHORS McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J., Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B., Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C., Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C., Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T., Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R., Waterston,R. and Wilson,R. TITLE The Washington Univ. Nematode EST Project, 1999 JOURNAL Unpublished COMMENT Contact: Mitreva M WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library constructed by: Irina Ronko and Dr. Makedonka Mitreva Putative full length read The vector to vector length is 459 Seq primer: SL1 primer. FEATURES Location/Qualifiers source 1..453 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1" /dev_stage="Mixed" /lab_host="GC10" /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI; Site_2: 3': EcoRI; The library was constructed by Irina Ronko and Dr. Makedonka Mitreva (mmitreva@watson.wustl.edu) at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Radopholus similis total RNA was provided by Dr. Godelieve Gheysen of Ghent University (lighe@gengenp.rug.ac.be)." ORIGIN 1 gaaacatgaa caagtattgc ctttgcatgt ttacgaggat gcacgagtta aaaacgccca 61 tcaggaggaa tatttgcatg agcgctgtac aacataaagg acactcgcat tggcagaatg 121 tggccaaaac aaaggcaaag gtggacctag aaaagaacaa agccttgaat gagatggtga 181 ggaagatgaa gaaggcagtg gaaggcggga actttgatcc agcacgaaac ccaaagttac 241 aggatatcat gaatgacatg agaaaacaat cgctttcgat ggaaacattt cacaaacggc 301 tcgccaaatt caaggacgag gcagcagctt catcgaaatc acaagaatag agggtaggag 361 acgggtaaca gtagcttatt gttcaattat cttaaattcg ctgttttatc aaaggcgaag 421 ttaataaaac atcattaaaa aaaaaaaaac aaa //