LOCUS       CO897611                 441 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03h07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YQD9_CAEEL Q09490 HYPOTHETICAL 14.8 KD PROTEIN
            C32D5.9 IN CHROMOSOME II. [1], mRNA sequence.
ACCESSION   CO897611
VERSION     CO897611.1  GI:51237401
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gatcaaaatg aagtggacct acaaggagga gaacccgttc gagaagcgac gcgccgaggg
       61 cgagaaaatc cgccgaaagt accctgaccg aattcccgtg attgtggaaa aggcgccgaa
      121 gtcgcgtctg cgcgacctgg acaagaagaa gtacctggtg ccgtccgagc tcaccatagg
      181 ccagttttac ttcctcatcc gcaagaggat ccacctccgc cccgaggagg cactgttctt
      241 cttcgtgaac aacatcatcc cgcagaccat gaccacaatg ggccagctat atcaggagca
      301 tcacgaagag gacctcttta tgtacatcgc ctacagtgat gagaacgtgt acggcggcgg
      361 agaggaagat gggaagaatg cgcttgagtg ctaatcatca aaaacctaga atcaaatgat
      421 ccgagcgtgt gtaccttttt t
//