LOCUS       CO897610                 608 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03h06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YQF4_CAEEL Q09269 HYPOTHETICAL 16.0 KD PROTEIN
            C34C12.4 IN CHROMOSOME III. [1], mRNA sequence.
ACCESSION   CO897610
VERSION     CO897610.1  GI:51237400
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aattcatagt caactgctac acaaatgtcg gatccgtgcg aatgcatctt caaccatgaa
       61 gcgattatgc gacggattct gtctatcttg caagaagccc aaaccgattg caccaacacc
      121 gaatgttcga ccgggccttc cgatgctggt gctggcggtt cgaacatgat gctcattgcc
      181 atggcttggg gactgttcgc gatgttcatg ttcatgatgc gtccggcctc gatgcgcagc
      241 tcgaccgggg gaaacgaggg tgacgctgca caggagaagg caccttccaa ttcgtccagc
      301 aataacaacg acaacaacgg accgcctccc ggtgatgtgt tctgagttgg ccgcggagaa
      361 acacggtgct tgcgcgaagt gcccggaccc ggatgaaaaa aaaccaaaca catcttcgtc
      421 ccttcatctg tccccaccac ttctcaaatt gggtaacatg tacagcaacc aaaactatcc
      481 cggatattca aaatcacccc tagttagatg gatttagtta cgcactttcc gtccacccca
      541 aatccaagaa attgattcta tttatttttt gttctcctgt ccagtctaca catttcctga
      601 atactaaa
//