LOCUS       CO897607                 508 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03h03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL21_CAEEL P34334 PROBABLE 60S RIBOSOMAL PROTEIN
            L21. [1], mRNA sequence.
ACCESSION   CO897607
VERSION     CO897607.1  GI:51237397
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cacctgttct gaagcaaaat ggtcaacccc aaaggatacc gccgtggcac ccgctacatg
       61 ttcgcgaggc cgttccgcaa gcacggcgtg gagccgctgt ccaccttcta ccgggtgtac
      121 aagcgtggcg acatcgtcac ggtcaagggc aacggcgcct tccaaaaggg catgccgttc
      181 aaggcctacc acggaaagac tggccgagtg ttcaacgtga ccaagcatgc gctcggcgtt
      241 gtggtcaaca aacgtgttcn caatcgcatc atcccgaagc gaattaatgt tcgcgtggag
      301 cacgtcaagc catctggctc ccgctcggag ttcttgcgcc ggtgcaaggc caacgaccag
      361 cagaagaagg accataaggc cggaaatgcg ccatatgttc cgctgcgccg caaaccgcag
      421 gaaccaagag gcgctcatat ggtcaagctg aacacgacgc acatcgaaga gctggcgcca
      481 gtgaaatacg aaattgttgc ttgagtga
//