LOCUS       CO897600                 493 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03g08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q18185 Q18185 SIMILAR TO C. ELEGANS PROTEIN
            C25H3.9. [1], mRNA sequence.
ACCESSION   CO897600
VERSION     CO897600.1  GI:51237390
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 437.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgtcggtgct taacaattgg gcccaactgc gacgcacttc acccccaaat ttttgtgcaa
       61 tgaactggcc aaaggtcgaa tctcacttcg agcgcctacg ctacagatag gccaaggagg
      121 agtttcagcg ctcattgtgc gacatggtca cggacacgtg acgcgcaggc gatatgggca
      181 gatggtttgg aacaaaacca aggactgttg gcatttctat atactcggca ttggcgttta
      241 tcccgcattt ttgatcctat ttctgacgca cgtttattac gggccctgtg aactcactga
      301 atatccaaag aaaggcgaac aaccaccacg ccattggcag ttcgagcgga ctccgttgcg
      361 ccaattttgg agcaaatggg tcacttatcc tgatatgcga taccacgaga gcaaccttgc
      421 gttctttgcg cgttgcgaaa acaaaaatag atggcgacgt ctccaacgac gcgtggaaca
      481 cttgcaagga gag
//