LOCUS       CO897595                 545 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03g03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL1X_HUMAN Q02543 60S RIBOSOMAL PROTEIN L18A. [1],
            mRNA sequence.
ACCESSION   CO897595
VERSION     CO897595.1  GI:51237385
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 538.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 caatgcctat taaagcatca ggagaaacgt tgaagaattt atcgtagttg gtcgcaaact
       61 tccgacggag aatgaccaaa atccagcact gtataagatg gttatctttg caagcaacca
      121 cgttgttgca aagtcccgct tctggtattt caccagcatg ttgcgtcgtg tgaagaaaac
      181 tcatggtgaa atcatctctt gcactgagtc attcgagaag aagcctggct ctgttaaaaa
      241 ctttggcatc tggcttcgtt atgactctcg aaccggccac cacaacatgt atcgcgagta
      301 ccgcgatgtt actgaggcag gtgcagtaac tcagtgttat cgtgatatgg gtgctcgtca
      361 tcgtgcacaa gcagaccgca tccaaattat caaggtccag acaatcaaag tggaggactg
      421 caagcgtgct ggtgtgaagc aattccacat ctcgaagatc cggttcccgt tgccgcatcg
      481 cgtgaatgcc aaacggcgcc agatggctcc attcaccact cagcgtccga agacccactt
      541 tgctt
//