LOCUS       CO897594                 449 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03g02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YQ5A_CAEEL Q09253 HYPOTHETICAL 32.0 KD PROTEIN
            C16C10.10 IN CHROMOSOME III. [1], mRNA sequence.
ACCESSION   CO897594
VERSION     CO897594.1  GI:51237384
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aatgccaatc aatgcccgtt cccttcacta tgttttccga attgccaacc gagaggcgtc
       61 ctacaagttc tacaccgatc tgctcggaat gaagatcctt cgtcatgagg agttccacga
      121 accatgccag gcttcgtgca atggcccgtt cgatgggatg tggagcaaga cgatggtggg
      181 ctatggcagt gaggaggacc acttcgtgct ggagctgacc tacaactacg aagtgcactc
      241 ctacgagttg ggaaacgatt tcgggggaat ccacatcgag tcggaggacg tgttccagag
      301 agtggcggcg gaggcgcagc gacagcccga cgggactctg gcgctgagag accccgacgg
      361 ccacgcgttc ttcatcggga ggggctccgc caaacacccc gtcaccaggg tgtccgtgaa
      421 cgttgtcgac ctggccaaat ccaggggtt
//