LOCUS       CO897592                 529 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03f12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:1434_CAEEL Q20655 14-3-3-LIKE PROTEIN 2. [1], mRNA
            sequence.
ACCESSION   CO897592
VERSION     CO897592.1  GI:51237382
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 458.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 acttgcaccg aactttaatt ttattcgctc tcctaatttg accgatcgca acaaaatggc
       61 cgagaacaag gaggaactcg tgcagcgtgc caagctggcc gaacaggctg agcgctacga
      121 cgacatggcg caggcaatga agaaggtgac cgagatcggc gcagagctga gcaacgagga
      181 gcgcaaccta ctgtcggtgg cctacaagaa cgtggtcggg gctcgccgct cctcgtggcg
      241 cgtgatctcg tccatcgaac agaagaccga gggcgccgag aagaagcagc agatggccaa
      301 ggagtaccgc gagaaggtgg agggcgagct gcgcgacatc tgccacgacg ttctggatct
      361 gctggacaag ttcttgattc cgaaggccgg aaacccggag tccaaggtgt tctacctgaa
      421 gatgaagggc gactactacc gctacctggc tgaggtggcc cccggagacg accgaagcat
      481 cgttgtggga gatttttatc aaggctacca gggaggtttt ttgatatcc
//