LOCUS       CO897589                 638 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03f09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:P90860 P90860 F36A2.7 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897589
VERSION     CO897589.1  GI:51237379
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tttgccttct agagtcttaa tttttactcc acatatttgt acttctggca gagctcgaac
       61 ttcgaaatga tcccaatttc taaattggct tcaccactcc acccagcctc ttcgggtgcc
      121 attctccgtg ttttcgcgat tcgaaaattt gccagtgctg ttttggaacg gccgccaaag
      181 gctaagatcc aactgaaacc accaccaggc gagaagggct acatgaacta tgaacgtgac
      241 ttttcacgcg aatcacgtta tacaaagcca cataagaaga ccgacacatt tataagattt
      301 gtaacattta acgctcgcca tcacatggag ctctggccga tcgtatttct ctgcggtgtc
      361 tcttgttgcc tagtcgtggg tatagcctac ataagttttg aaaaagtgga agtctggctt
      421 gacagaagca ccaagacccc accatgggat tgggcgcgca ttcgtgacaa atactggaga
      481 atgcaaacag tatttttcga tccacttggc gggacccacc agcgtataga aatcatggag
      541 aagctacagg atgagatgtt ggaggcggcc aagcagcgtg gaacccgcga tgccggtggc
      601 tacattgtta ctaactaaaa cgggaaggcc agcaaagc
//