LOCUS CO897587 335 bp mRNA linear EST 12-JAN-2011 DEFINITION pe03f06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA 5' similar to TR:Q20588 Q20588 F49C12.11 PROTEIN. [1], mRNA sequence. ACCESSION CO897587 VERSION CO897587.1 GI:51237377 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 335) AUTHORS McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J., Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B., Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C., Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C., Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T., Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R., Waterston,R. and Wilson,R. TITLE The Washington Univ. Nematode EST Project, 1999 JOURNAL Unpublished COMMENT Contact: Mitreva M WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library constructed by: Irina Ronko and Dr. Makedonka Mitreva Putative full length read The vector to vector length is 437 Seq primer: SL1 primer. FEATURES Location/Qualifiers source 1..335 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1" /dev_stage="Mixed" /lab_host="GC10" /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI; Site_2: 3': EcoRI; The library was constructed by Irina Ronko and Dr. Makedonka Mitreva (mmitreva@watson.wustl.edu) at Washington University, St. Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis cDNA PCR products of size >400 nucleotides containing SL1 on the 5' end and oligo(dT) on the 3' end were non-directionally cloned into pCRII-TOPO(Invitrogen) following the Topo TA cloning protocol. Radopholus similis total RNA was provided by Dr. Godelieve Gheysen of Ghent University (lighe@gengenp.rug.ac.be)." ORIGIN 1 gcattaatta acttaatcgc cgagattcta atcgcgtccg aaaagcgcac aatgtctggc 61 cgtcaaggag gcaagctaaa accgttaaaa caggcgaaga agggcgacaa agtggaaact 121 gaggaggatg tggaattcaa aaagaagcag atggaggagc aaaagaagct gaaagagatg 181 acgcagaagg cagcgcaaaa gggcccaatg gctgctggtg gaatcaagaa gtcgggcaag 241 aaataaagac gaaagccttc atgagaccaa gtgatacagc attgacgata agcggaacat 301 tcgactgtca ttcaagatta aactattccc ccata //