LOCUS       CO897580                 524 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03e08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9U1P3 Q9U1P3 UBQ-2 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897580
VERSION     CO897580.1  GI:51237370
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ctgattctgt cgactgaata tcaccaattc taaaatgcaa atatttgtga aaacactgac
       61 cggaaaaact atcacactcg aagtcgaggg gagtgacaca atcgaaaatg taaaagcgaa
      121 gatccaggac aaagaaggaa ttcctccgga ccagcaacgt ctgattttcg cgggaaaaca
      181 gttggaggac ggacgtactc tggcggatta caacattcag aaggagtcca ctttgcactt
      241 ggtgctgcgt ctccgcggag gaatcattga gccgtcgctg cgcatgttgg ctcagaagta
      301 caactgtgac aagcagattt gccgcaagtg ctatgctcgt ttgcctccgc gtgcaaccaa
      361 ctgccgaaag aagaagtgcg gtcactccaa cgacctccgc gtcaagaaga aacttaagtg
      421 aagaagcaga tggattggcg gaagagccga agatgtccat cgctattttg agctgttaat
      481 cgttcaccat tgttgacgta taaattggat gtaaaaccaa aaaa
//