LOCUS       CO897573                 489 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03d11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to WP:CE00756 C07H6.2, mRNA sequence.
ACCESSION   CO897573
VERSION     CO897573.1  GI:51237363
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 508
            Seq primer: SL1 primer
            High quality sequence stop: 472.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cccgcctccg cctcaccatg tccagcacaa caaaacagtc cgagaaatgc catcaccact
       61 cgaactgaga ctgtcactct cgagcgtacg tcggatgagc aagttgtact cagattggag
      121 tcgccggcag tgccagacac caggccaaga gtgcagtgga caactgacac agtggacaac
      181 gagcatttgg gcaagaaaaa gtccaaatgc tgctgcatct acaagaagcg tcgcaactgg
      241 gacgaaagcg atacagagga cgatgaatgt gagagtgagc actgtcgcgg ccacgtggag
      301 aagcacagac gcgaccaagg aggagatccg ggcgctggcc cttctggcga tggtgggccc
      361 gcagttccct catgatctcg ctgagcgagt tcattggacc aattccgatc cagcttcttt
      421 taactctgtc aattcttccc aaggaatttc acaataaata ttgtttatat canaaaaaaa
      481 aaaaaaaaa
//