LOCUS       CO897566                 466 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03c11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:BC10_HUMAN O60629 BLADDER CANCER 10 KD PROTEIN,
            mRNA sequence.
ACCESSION   CO897566
VERSION     CO897566.1  GI:51237356
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 516
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aatttacaat ttaccattaa aatgtattgt ctacagtggt tgatgccggt tttactaatc
       61 ccgaaacaac ttattcatcc tacttttcta attgatcaag cgctgtttct ttggttctat
      121 ataatcggct ttgtgctcga gaggaggcct tgctacattt gtttgatcat ctttgctgtc
      181 accgtttttg taatttgcta cagcgatgaa gagcggtgtg ttctatggcc agtttgcgag
      241 acggtcttag aaggagaaat gtgcaggcgt gcatatgatg ccgaaacagt gacggtttga
      301 tgatgaatgc attaagcttt gattttttaa actttgtaca accatcacgg gttcgtcgtc
      361 atcttttcaa tcaaatacta gcagagatat tatcgcacaa gtaacattct gattcttgca
      421 aataccttat tttataaatt ttcgaacgtt aaaaaaaaaa aaaaaa
//