LOCUS       CO897559                 536 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03c03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YQ5B_CAEEL Q09254 HYPOTHETICAL 15.4 KD PROTEIN
            C16C10.11 IN CHROMOSOME III. [1], mRNA sequence.
ACCESSION   CO897559
VERSION     CO897559.1  GI:51237349
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 649
            Seq primer: SL1 primer
            High quality sequence stop: 521.
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttattcatta gcttacataa aaatggttcg aagacgctct gcttctccga gggccagtgc
       61 tcccgtgcgc tcagccgcgc cggctcgtgc cccggctcct gctgccgcgc ctcctcgtcc
      121 ggctggcgct ccgcctgctg gcgcaacccc gcaaggccct ggccttttcg gccaaatggc
      181 tgcaacagcg ggaggtgtcg caatcggttc ggctgtgggc catgccatcg gaaacatgtt
      241 ctctggcggc gctgcgcgtg aggccgctcc agccgcagaa aacaacgctc aaggctatga
      301 gaagcaacag ccgcagcaat actcacagcc gtgcgagttc gaatggcgcc aatttcttga
      361 ctgttcccaa aaccaatcgg atgtcagcgt gtgccagggc tacaacgact tgttcaagga
      421 gtgcaaggct cgtcaaggcc tctaaatgga agggtctggc ggctatcgag attgattgaa
      481 agctcgagca atgaaaccgc gatacatatt tagttggatc anattacacc ttcacg
//