LOCUS       CO897551                 598 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03b05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS8_CAEEL P48156 PROBABLE 40S RIBOSOMAL PROTEIN
            S8. [1], mRNA sequence.
ACCESSION   CO897551
VERSION     CO897551.1  GI:51237341
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Trace considered overall poor quality
            Seq primer: SL1 primer
            Sequence considered overall poor quality.
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gggtatctcc cttgacaggt ggccctcgcg cattagaacc ggggggcaac gcattcccat
       61 tcacaagaaa ctaaattatg acatggcact tcgaactgca tttactaaga ttggtcccaa
      121 taggatccac ttggtccgtt gccttggtgg aactatctaa cgccgaggac tgcgttagca
      181 cgcttgaaac ttcccttggt ccgcttatgg gagcacccgc cagatcctta ttttctgctc
      241 tgagtagcat gcttcgcact cctatctgtt ccgcacgtcg accctcgtcc agggctccat
      301 cctcgccatt gactctcttc cgttccgcca ctggtaacag gcccactatg cgctgccttt
      361 ggctcgcaag aagggacaaa agttgaccga cgaaaaagaa gcgcgcatca atggggcaca
      421 aaagcgttcc aagtcgatcc tgaagaagta ctcagagcgc caaaagacag ccgccgtata
      481 gccgcatctg cttgagcagt tccaatctgg acgtctcctt gcttgcatca gctccatacc
      541 cggacatagc ggacgccttg acggctatat cctgcagggc acagacctcc attcctac
//