LOCUS       CO897550                 564 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03b04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS4_YARLI O59950 40S RIBOSOMAL PROTEIN S4, mRNA
            sequence.
ACCESSION   CO897550
VERSION     CO897550.1  GI:51237340
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 535.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 agcaaggcta atcatcacaa tcattaaaag cgaataatgg cacgtggacc gaaaaagcat
       61 ttgaaacgtt tggcggcacc caagaactgg atgttggata agttgggcgg cgtgtttgct
      121 ccacgtccta tgtgcggacc acacaagctt cgcgagtcgc tcccgctcat tctttttctg
      181 cgaaaccgtc tcaagtacgc ccagtcctat aacgaggcgc gcatgatctg caagcaacgt
      241 ctaattaaag tagacggcaa ggtgcgaacg gagatgcgtt tcccggctgg attcatggat
      301 gtaatctcga ttgagaaaac tggcgagact ttccgtttgt tgtacgatgt gaaaagccgc
      361 ttcacagtcc atcgcatcca aaaagtggag ggacaataca aattgtgcaa ggtggtcaag
      421 caagcgatcg gtcccaagca ggtgccatac attgtgaccc atgatgcacg caccattcgc
      481 tacccggacc cgcatctaag agtcgatgat accgtggtga ttgatattgt gagcggaaaa
      541 attagcgact acgtgaagtt cgaa
//