LOCUS       CO897548                 697 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03a11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q21924 Q21924 R11A8.2 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897548
VERSION     CO897548.1  GI:51237338
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 688.
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cctctcccct ctgtttataa tcaccaatca gcatattttt atcaattaaa aaacagcccg
       61 cgcaaaaaca acaatgcatc caactactcc atcacctgat tcttcaaatc cgtccattgt
      121 tgatgcaagt gaagagattg tcgcaaaaga ggatgttatg attgagactt ctaaaacact
      181 atcttcgtct tcgactgccg aaagcggggg gaaaatctca ttcagcgtta ccaaaaggca
      241 aacgaccaga atagttacga caaaaattcg acttgatcgt gcaagagatg ctgctgaaga
      301 ggaggaggag gagcaacaaa acaggaagag aggcgtcctc agcttggaag atggcgtttg
      361 catagatgcc gaagcgccaa acaaaaaacg tgagaagaag gtgtttgtca tcccggtagt
      421 tcaaacgtcc agccaattgc aaatcgagcg actgaatagg ttggtggatg aaggcactgc
      481 cacagaatca gatcgtgctc gtctcactct attgctagaa agtttgggag aagtacctac
      541 tcaattaaaa aatgagaaat tcacacttcc atctggcgat attcaaactg gaccgacaac
      601 cgtgatagca gatgaaaccg atccagacta tgaaagtatg cctattggcg aatttggtct
      661 cgctttcttg cgtggttgtg gctggaaaga tgaaaat
//