LOCUS       CO897545                 519 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03a07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:THI2_RAT P97615 MITOCHONDRIAL THIOREDOXIN
            PRECURSOR, mRNA sequence.
ACCESSION   CO897545
VERSION     CO897545.1  GI:51237335
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 506.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tttcgcgcga tttcttcgct gatattcaaa cgcactgcct ttcgagcgcc aaccggcatc
       61 ttgtgcagaa aattattggc acaagcatga taattggtgg agccgccctt tgccgacgca
      121 cttttgcagc cccagccccc catcgggaca cggtattcga ggtagaatcg gacgaggact
      181 tcgagaatgg ggtataccat gccgaaaaac cagtgctcat gcagttttac gccgattggt
      241 gtggaccctg tcagaatttg gcaccgaggt tgattgccaa agtgaatgga caggatggga
      301 aggtattgct agcgagagtg aatgtagagg gttccgccgg atttctcgcg gaacagtttg
      361 atgtaagctc gattcccact gtgatgtgct ggctgcgagg agaggtggtt gaccgttttg
      421 agggcgacgt ggaagacacg aagattgacc aaatcatatc caaattggtg gaatatcaaa
      481 ctggaaatga atgataaagg gcttgaacag cccaaaaaa
//