LOCUS       CO897543                 519 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe03a03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to contains element TAR1 repetitive element, mRNA
            sequence.
ACCESSION   CO897543
VERSION     CO897543.1  GI:51237333
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcgacagcta tttgttgttc tccgtcgcac aactggaaaa atgagcgcaa cagagcagca
       61 gacacaaaag cggcgcgccg aggagaatgg cgacgaagaa gtcgcaacaa aggcgcagaa
      121 agtcgacaac ggaggcgctg taaagggcgc aaatgacgtg aacgccaaca gcaacaagct
      181 gaaagccaaa gcggtgaata acgaaaaggt agccgccgag gaggagacgg aagagaacga
      241 gggcgaggag gagagcgagg gcgagtncgg ggcaggagag gacgacgacc tgggcgacga
      301 ggcggactcc ggggaggagg acgctgacga cgaggaggac gatgaaggcg cgggtggaga
      361 cgacgacgag gagggagacg atgcagaaga ggacgcggaa ggggacgaag aggaggagga
      421 agaggaagaa gaatgagctg gccggcgaca ctttaagcac ccaaaccaaa gaatgggaca
      481 tcgagacgaa atttggaatg tttgcgattc gtcgtcgtt
//