Technology

Centre : Central Potato Research Institute(CPRI)
Name of the technology :
Identified novel genes for late blight resistance in wild Solanum species by allele mining and transgenics development through overexpression of R genes

Inventors

1. Jagesh Kumar Tiwari
2. BP Singh
3. SK Chakrabarti
4. Vinay Bhardwaj
5. Sundaresha S
6. Sanjeev Sharma
7. Shashi Rawat

Procedure / Summary :

a) Identification of R-genes in wild potato species  Identified novel 17 RB-homologous gene fragments in 11 wild species (S. chacoense, S. pinnatisectum, S. polyadenium, S. trifidum, S. cardiophyllum, S. lesteri, S. huancabambense, S. verrucosum, S. jamesii, S. polytrichon and S. stoloniferum) from total of 44 wild potato species for late blight resistance. The isolation and characterization of 17 RB-homologous gene fragments (NCBI sequence No. KJ472305-KJ472320 and KJ610025) from wild potato species may serve as an important genomic resource for the novel gene discovery in late blight resistance breeding programs. b) Developed potato mapping populations for genetic studies Two new diploid mapping populations were developed by crossing wild species viz. S. acauli × S. verrucosum (570 true potato seeds), and S. polytrichon × S. verrucosum (1390 true potato seeds) for mapping studies of late blight resistance. c) Developed gene constructs and putative transgenics for late blight resistance Isolation of allelic variants/homologues of the late blight resistant gene Rpi-pta1 from wild potato species S. cardiophyllum and S. microdontum. Confirmation of the gene insert by restriction digestion of the binary vector pRI101-AN with enzymes KpnI-EcoRI.

Applications

Management of late blight through transgenics development and also through developing new potato genotypes through marker-assisted breeding.

Advantages

Late blight resistant genotypes would be developed.

Status of Commercialization : Not applicable

Publications

1.  Jagesh Kumar Tiwari, S Devi, S Sharma, P Chandel, S Rawat and BP Singh (2015) Allele mining in Solanum germplasm: cloning and characterization of RB-homologous gene fragments from late blight resistant wild potato species. Plant Molecular Biology Reporter 33:1584–1598.
2.  Jagesh Kumar Tiwari, S Sundaresha, BP Singh, SK Kaushik, SK Chakrabarti, V Bhardwaj and P Chandel (2013) Molecular markers for late blight resistance breeding of potato: an update. Plant Breeding 132: 237–245.