Centre : Central Potato Research Institute(CPRI)
Name of the technology :
Development of dsRNA based fungicide for management of potato late blight
Developed an in vivo expression system to produce large amounts of dsRNAs encoding P. infestans genes in bacteria with a view to provide a practical control of late blight disease in potato. Designed the gene specific primers tailed with T7 promoter sequence and cloned into normal cloning vector pTZ57R/T. Positive clones were subsequently subjected to induce synthesis of dsRNA in T7 express cells by using IPTG. IPTG induced bacterial dsRNAs promoted specific interference with the Phytophthora infection in potatoes. Furthermore, we have demonstrated that it is easy to obtain crude extracts of bacterially expressed dsRNAs which are equally effective protecting potato against P. infestans infections when sprayed onto plant surfaces along with emulsifying agents by a simple procedure. Late blight symptoms were significantly lower when plants were sprayed with bacterial lysate before pathogen inoculation.
dsRNA based fungicide formulation for management late blight.
Eco-friendly management of late blight.
Status of Commercialization : Applied for patent