Centre : Central Potato Research Institute(CPRI)
Name of the technology :
Rhamnolipid bio-formulation for late blight management
Characterized microbes for their bio-surfactant properties and found five isolates of Pseudomonas aeruginosa promising for bio-surfactant production. Testing of biosurfactant efficacy against P.infestans was done through food poison test at different concentration (0.1µl/L, 0.5µl/L, 1µl/mL, 2µl/mL and 5ul/mL) and cent per cent radial growth inhibition was observed at all tested concentrations except at 0.1µl/L. The characterization of biosurfacatnant was done by TLC plate which showed presence of glycolipids in the biosurfactant. Analysis of crude biosurfactant of Pseudomonas aeruginosa was carried out by high performance liquid chromatography and mass spectroscopy and compounds were identified as rhamnolipid congeners based on their molecular formula and molecular weights. Rhamnolipid secreted by P.aeruginosa was collected from the methods described by Kim et al. (1997) for formulation development. The rhamnolipid based formulation was developed in different solvents. Finally cyclohexanone was selected. The detached leaves experiment was conducted to evaluate the phytotoxicity and biocontrol effect of rhamnolipid based formulation on different concentration starting from (0.0025 to 0.5%). Result revealed that phytotoxicity was not observed up to 0.25% concentration and this formulation was as effective as contact fungicides.
Management of late blight of potato through non-chemical approach.
Eco-friendly management of late blight.
Status of Commercialization : Not applicable