Centre : Central Potato Research Institute(CPRI)
Name of the technology :
Identifications of QTLs for late blight resistance
Developed mapping population (126 F1) for identification of horizontal resistance genes to late blight using S. chacoense (resistant) S. spegazinni (susceptible) species. The population was genotyped using 23 selective AFLP primer combinations and one SSR marker. A total of 4,002 loci were amplified out of which 2,698 (67%) were polymorphic. Molecular linkage maps of S. chacoense and S. spegazinii were prepared containing 208 and 247 markers, respectively. The data so generated could resolve 12 linkage groups. Based on AUDPC dataset of in vitro whole plant assay, interval-mapping analysis identified two QTL (LOD> 2.5) located on linkage groups IX and X, which explained 14.70 and 3.40% variation, respectively. The study revealed the presence of potential new genetic loci in this diploid potato family contributing to quantitative resistance against late blight. Consequently, a gene (QTL) was amplified and sequenced from the mapping population (SC-34) of about 1100 bp size. Further, to make a gene construct in the Binary vector pRI201 at restriction site of NdeI and SalI, target PCR product and vector were restricted with the enzymes. Finally, gene constructs were prepared by ligation of the restricted insert and the vector for the transformation to the commercial potato cultivars.
MAS for late blight resistant breeding.
Development of late blight resistant varieties.
Status of Commercialization : Not applicable