Centre : Central Potato Research Institute(CPRI)
Name of the technology :
Multi gene siRNA knock-down applications for late blight resistance
The best approach to create mutant for non-pathogenicity in Phytophthora is to construct vectors for the simultaneous expression of multiple siRNAs targeting more than one gene, which would significantly inhibit the pathogen life cycle. All cluster of genes involved in the life cycle of the Phytophthora or signalling process could be knocked down at once, especially in the study of complex signal transduction cascades. Based on this hypothesis, we targeted the various cellular processes of Phytophthora infestans including sporulation, signalling process and protein synthetic machinery of the cell to create the mutant for non-pathogenicity. Using siRNA target finder software, selected the effective siRNA of the targeted gene of interest and stacked all siRNA in a single synthetic construct driven by T7 promoter on both 5’ and 3’ end. The efficacy of siRNA has been assayed/checked using in vitro dsRNA technology through detached leaf assay against P. infestans. Results were found highly significant and this would give a proof of concept for development of RNAi based fungicide as well as resistant genotypes through RNAi technology.
Development of RNAi technology for durable resistance against late blight.
Development of resistant varieties.
Status of Commercialization : Not applicable