Centre : Central Potato Research Institute(CPRI)
Name of the technology :
Development of P. infestans Pol gene RNAi construct
The dynamism in P. infestans is mainly due to the movement of transposable elements. Nearly 33% of the genome is covered by transposable Long Terminal Repeats LTR/Gypsy-Ty3 element, which is almost1/3rd of the genome. Gypsy-Ty3 is well characterized and is independent transposable element and code for its own tranposase enzyme which can be targeted for silencing in P. infestans. The strategy adopted was to construct inverted repeat cDNA fragment of Phytophthora Gypsy like element GypsyPi-1a gene intervened by potato granule bound starch synthase (GBSS) intron (iIR-GypsyPi-1a). Transcription of iIR-GypsyPi-1a gene construct in the transgenic potato background would generate hairpin (hp) Gypsy element Pol transcript, which will induce formation of siRNA causing silencing of Pol gene expression. 307bp cDNA sequence of pol was included for construction of sense and antisense fragment of iIR-GypsyPi-1a. The iIR-GypsyPi-1a gene construct under the control of constitutive promoter CaMV 35S on MCS of binary plant transformation vector pRI101. A total of 300 internodal stem cuttings of Kufri Bahar (LB susceptible cultivar) have been used for Agrobacterium mediated genetic transformation and putative transformants will be used for bioefficacy studies.
Understanding pathogen dynamism and development of late blight resistant varieties.
Technology for management of late blight of potato.
Status of Commercialization : Not applicable