Technology

Centre : Central Citrus Research Institute, Nagpur
Name of the technology :
Development of talc-based bioformulation of Trichoderma harzianum strain NRCfBA-44 for managementof Phytophthora root rot of citrus

Inventors

  1. Das, A. K.
  2. Kumar, A.
  3. Nerkar, S. G.
  4. Thakre, N.
  5. Landge, P.
  6. Bawage, S. S.

Procedure / Summary :

A talc based formulation of a native strain (NRCfBA-44, isolated from Vidarbha region of Maharashtra soil) of bio control fungus Trichoderma harzianum, was developed for the management of Phytophthora root rot of citrus. Briefly, fungal mycelial disc (6 mm diameter) was inoculated in 100 ml PDB. Conidiation was counted after 7 days and the mycelial mat along with conidia from PDB were mixed thoroughly with autoclaved talcum powder pre-treated with 0.5 % CMC. The mix was air-dried in a laminar flow hood and the colony forming units were counted; it was packed subsequently in a poly bag and sealed. The sterilized talc powder bioformulation, containing minimum population of 107 CFU/gram, can be stored up to 8 months without significant reduction in viability. Application dose: At the orchard: 100 g of formulation/ mature plant with carrier material of 1 kg well-decomposed FYM. At the Nursery: 20 g of formulation / polybag of 2 litre capacity. Time of Application: Two applications per annum; First, during onset of monsoon (June-July) and second, post- monsoon (Sept-October).

Applications

Biological control of Phytophthora root rot of citrus, This formulation can be used in Integrated Disease management (IDM) programme for Phytophthora root rot of citrus as well as in organic citrus cultivation.

Advantages

This is an eco-friendly component of management. This product is useful for the management of Phytophthora root rot disease of citrus. It also promotes growth of citrus plants.

Status of Commercialization : Not applicable

Publications

  1. Das, A K, Kumar A, Nerkar S and Thakre N. (2016) Biological control of Phytophthora root rot and gummosis of citrus with novel Trichoderma strains native to Vidarbha region of central India. 14th International Trichoderma and Gliocladium Workshop (TG2016) Principles to Practice held at College of Agriculture, Nagpur, Maharashtra, November 27-30, 2016. pp. 31-32.
  2. Das, A K, Kumar A, Nerkar S, Thakre N. and Landge P. (2015) Trichoderma harzianum native strain NRCfBA-44: isolation, characterization and formulation development for biological control of Phytophthora root rot of citrus. National Symposium on sustainable citrus production: Way Forward held at CCRI, Nagpur, Maharashtra, November, 27-29. pp. 84-85.
  3. Das, A.K., Ashok Kumar, Nerkar, S. and Bawage, S. (2012) Trichoderma harzianum strain NRCfBA-44, a novel biocontrol agent for the management of Phytophthora root rot in citrus. Paper presented at the Fifth Indian Horticulture Congress (6 -9th November, 2012), held at Punjab Agricultural University, Ludhiana, Punjab.