A Novel Antifungal Furanone From Pseudomonas aureofaciens, a Biocontrol Agent of Fungal Plant Pathogens
Paulitz. T Nowak-Thompson. B Gamard. P Tsang. E Loper. J
Journal of Chemical Ecology ; 2000 [Vol.26] Pages:1515-1524
Abstract
Pseudomonas aureofaciens (c P. chlororaphis) strain 63-28 is a
biocontrol agent active against many soil-borne fungal plant pathogens and
shows antifungal activity in culture assays. 3-(1-Hexenyl)-5-methyl-2-(5H)fur-
anone was isolated from culture filtrates of this bacterium. The purified
furanone showed antifungal activity against Pythium ultimum, Fusarium
solani, Fusarium oxysporum, and Thielaviopsis basicola. The ED50S for spore germination of these fungi were 45, 54, 56, and 25 mg/ml,
respectively. The compound also inhibited the germ tube growth of
Rhizoctonia solani growing from microsclerotia, with an ED50 of 61 mg/ml.
The compound is the reduced form of furanones previously described
from this bacterium: 3-(1-hexenyl)-5-hydroxy-5-methyl-2-(5H)-furanone and
3-(1-hexenyl)-5-hydroxymethyl-2-(5H)-furanone. This volatile antifungal fur-
anone has structural similarity to other antifungal furanones produced by
actinomycetes (Streptomyces spp.), fungi (Trichoderma harzianum), and higher plants (Pulsatilla and Ranuculus spp.). This is the first report of 3-(1-hexenyl)-5-methyl-2-(5H)-furanone produced by a bacterium.
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