FLASH-PCR Diagnostics of Toxigenic Fungi of the Genus Fusarium
Ryazantsev. D. Y Zavriev. S. K Abramova. S. L Evstratova. S. V Gagkaeva. T. Y
Russian Journal of Bioorganic Chemistry ; 2008 [Vol.34] Pages:716-724
Abstract
A test system for the diagnostics and identification of seven toxigenic fungi causing Fusarium head blight (Fusarium graminearum, F. culmorum, F. poae, F. sporotrichioides, F. langsethiae, F. avenaceum, and F. tricinctum) was developed using PCR. The identification of pathogen is based on the specific amplification of a DNA fragment of the gene of translation elongation factor 1 alpha (tef-1α) and subsequent detection of the results by the fluorescent amplification-based specific hybridization method. The system was tested on 38 isolates of different fungi of the genus Fusarium.
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