Bacillus spp. as biocontrol in an infested soil with Fusarium spp., Rhizoctonia solani Kühn, and Phytophthora capsici Leonian, and its effect on development and yield of pepper crop (Capsicum annuum L.). / Bacillus spp. como biocontrol en un suelo infestado con Fusarium spp., Rhizoctonia solani Kühn y Phytophthora capsici Leonian y su efecto en el desarrollo y rendimiento del cultivo de chile (Capsicum annuum L.)
Guillen-Cruz. R Guilar-Gonzalez. C. N Padron-Corral. E Hernandez-Castillo. F. D Gallegos-Morales. G Rodriguez-Herrera. R Reyes-Valdes. M. H
Revista Mexicana de Fitopatología 24 (2) Obregón: Sociedad Mexicana de Fitopatología ; 2006 [Vol.24(2)] Pages:105-114
Abstract
The objectives of this investigation were to determine the potential of four isolates (B1, B3, B9, and B13) of Bacillus and their mixture for biocontrol of root rot pathogens under field conditions, as well as their effect on development and yield of pepper. The application of Bacillus increased plant height 20% and yield 270%. Also, it decreased incidence 80% and severity of root rot 39% in comparison to the check. Bacterial identification was carried out by biochemical tests, Biolog badges, and polymerase chain reaction (PCR) with specific primers for the ribosomal gene 16S. Isolates of Bacillus B1 were identified as Bacillus amyloliquefaciens, B3 as B. licheniformis, and B9 and B13 as B. subtilis. In a phylogenetic tree, these Bacilli were grouped separately with other isolates of Bacillus.
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