Identity of Phytophthora associated with arecanut and its relationship with rubber and cardamom isolates based on RFLP of PCR-amplified ITS regions of rDNA and AFLP fingerprints
Chowdappa. P Brayford. D Smith. J Flood. J
Current Science ; 2003 [Vol.85] Pages:585-587
Abstract
ITS-RFLP and amplified fragment length polymorphism (AFLP) fingerprints were used to characterize Phytophthora species associated with fruit rot of arecanut in India. These were compared with isolates of P. meadii, causing leaf fall of rubber and fruit rot of cardamom, and P. palmivora isolates from cocoa and coconut. Reference cultures of P. arecae from coconut in Indonesia and P. meadii from rubber in Sri Lanka, Malaysia and India were also examined for comparison. The P. meadii isolates from arecanut, rubber and cardamom were found to be uniform based on ITS-RFLP and AFLP patterns. It is concluded that P. meadii is the main pathogen causing fruit rot of arecanut in India and there is no evidence of occurrence of P. arecae.
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