A simple in-vitro ‘wet-plate’ method for mass production of Phytophthora nicotianae zoospores and factors influencing zoospore production
Ahonsi. M. O Banko. T. J Hong. C
Journal of Microbiological Methods ; 2007 [Vol.70] Pages:557-560
Abstract
A simple in-vitro ‘wet-plate’ method for mass-producing Phytophthora nicotianae zoospores at ≥ 1.0 × 106 zoospores/ml is described. Temperature critically affected zoospore production; 22 °C was optimum, while 36 °C was completely inhibitory. Zoospores being the most important propagule of P. nicotianae, temperature of recycled irrigation water may be manipulated to reduce diseases in irrigated nursery crops.
Full Text