High-resolution mapping and analysis of the resistance locus Rpi-abpt against Phytophthora infestans in potato
Park. T. -H Vossen. E. V. D Jacobsen. E Vleeshouwers. V. G. A. A Hutten. R. C. B Eck. H. J. V Visser. R. G. F
Molecular Breeding ; 2005 [Vol.16] Pages:33-43
Abstract
Introduction of more durable resistance against Phytophthora infestans causing late blight into the cultivated potato is of importance for sustainable agriculture. We identified a new monogenically inherited resistance locus that is localized on chromosome 4. The resistance is derived from an ABPT clone, which is originally a complex quadruple hybrid in which Solanum acaule, S. bulbocastanum, S. phureja and S. tuberosum were involved. Resistance data of the original resistant accessions of the wild species and analysis of mobility of AFLP markers linked to the resistance locus suggest that the resistance locus is originating from S. bulbocastanum. A population of 1383 genotypes was screened with two AFLP markers flanking the Rpi-abpt locus and 98 recombinants were identified. An accurate high-resolution map was
constructed and the Rpi-abpt locus was localized in a 0.5 cM interval. One AFLP marker was found to cosegregate with the Rpi-abpt locus. Its DNA sequence was highly similar with sequences found on a tomato BAC containing several resistance gene analogues on chromosome 4 and its translated protein sequence appeared to be homologous to several disease resistance related proteins. The results indicated that the Rpi-abpt gene is a member of an R gene cluster.
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