Black shank resistant tobacco by silencing of glutathione S-transferase
Hernández. I López. Y Pujol. M Chacón. O Rodriguez. R Portieles. R Borrás-Hidalgo. O
Biochemical and Biophysical Research Communications ; 2009 [Vol.387] Pages:300-304
Abstract
A glutathione S-transferase gene was amplified from cDNA of Nicotiana tabacum roots infected with Phytophthora parasitica var. nicotianae. The gene was cloned in sense and anti-sense orientation to an RNAi vector for induced gene silencing, and reduced expression of the gene was detected by RT-PCR. A statistically significant increase in resistance of N. tabacum to infection following gene silencing was found for glutathione S-transferase-silenced plants compared with control plants. Some defense genes were upregulated in glutathione S-transferase-silenced plants during the interaction with the pathogen. This is the first evidence of the role of glutathione S-transferase as negative regulator of defense response.
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