Purification and characterization of elicitor protein from Phytophthora colocasiae and basic resistance in Colocasia esculenta
Mishra. A. K Sharma. K Misra. R. S
Microbiological Research ; 2009 [Vol.164] Pages:688-693
Abstract
An elicitor was identified in the fungus Phytophthora colocasiae. The molecular weight of the purified elicitor was estimated by means of gel filtration
chromatography and SDS-PAGE and was estimated as 15 kDa. Protease treatment
severely reduced its activity, allowing the conclusion that the elicitor is proteinaceous. Infiltration of a few nanograms of this proteinaceous elicitor into taro leaves caused the formation of lesions that closely resemble hypersensitive response lesions. The elicitation of the cells was effective in the induction of the activity of lipoxygenase. Cellular damage, restricted to the infiltrated zone, occurred only several hours later, after the infiltration of the elicitor protein. After few days, systemic acquired resistance was also induced. Thus, taro plant cells that perceived the glycoprotein generated a cascade of signals acting at local, short, and long distances, and causing the coordinate expression of specific defence. The obtained results give important information regarding the plant–pathogen interactions, mainly as subsidy for taro improvement against Phytophthora leaf blight.
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