Untitled Document
 

Untitled Document
Untitled Document
Full Text                                                                            Full-Text Online       
Mutagenesis study on amino acids around the molybdenum centre of the periplasmic nitrate reductase from Ralstonia eutropha
Hettmann. T      Romao. M. J      Diekmann. S      Siddiqui. R. A      Frey. C      Santos-Silva. T      
Biochemical and Biophysical Research Communications ;  2004  [Vol.320]  Pages:1211-1219
Abstract
Molybdenum enzymes containing the pterin cofactor are a diverse group of enzymes that catalyse in general oxygen atom transfer reactions. Aiming at studying the amino acid residues, which are important for the enzymatic specificity, we used nitrate reductase from Ralstonia eutropha (R.e.NAP) as a model system for mutational studies at the active site. We mutated amino acids at the Mo active site (Cys181 and Arg421) as well as amino acids in the funnel leading to it (Met182, Asp196, Glu197, and the double mutant Glu197-Asp196). The mutations were made on the basis of the structural comparison of nitrate reductases with formate dehydrogenases (FDH), which show very similar three-dimensional structures, but clear differences in amino acids surrounding the active site. For mutations Arg421Lys and Glu197Ala we found a reduced nitrate activity while the other mutations resulted in complete loss of activity. In spite of the partial of total loss of nitrate reductase activity, these mutants do not, however, display FDH activity.
Keywords
nitrate reductase
formate dehydrogenase
oxidoreductases
molybdopterin
ralstonia eutropha